Cytokinin is generally found in higher concentrations in meristematic regions and growing tissues. One report states that Coconut milk and water, which is the liquid endosperm, stimulate cell division. At least 20% of the cytokinin activity of coconut milk could be attributed to G3A2-ZR. Coconut water is a natural diuretic, meaning that it flushes out excess sodium and can help reduce the symptoms of those suffering from hypertension. These cytokinins can be found in coconut water and in the xylem sap of most plants. How often should you drink coconut milk? Education. This experiment is done with coconut water,water with tulsi leaves. The active. The answer is the option (c) Cytokinin Coconut water hosts an abundance of cytokinin, which helps regulate cell growth and cell division. Coconut water can even help lower blood pressure . Cytokinins (Letham, 1963) are plant growth hormones which are basic in nature, either aminopurine or phenyl urea derivatives, That promote cytokinesis (= cell division) either alone or in conjunction with auxin. Coconut water has been shown to stimulate shoot proliferation in many species of plants. The best CZE separation for the six cytokinin nucleotide standards was achieved using a 25 mM ammonium formate/formic acid buffer (pH 3.8) and 2% (v/v) methanol with an applied gradient separation voltage (25 kV for 32 min, and then a linear gradient to 30 kV in 5 min, finally 30 kV to the end of separation) in less than 60 min. I drill into the bottom center of the coconut and shake it till all the water is removed from the nut. Coconut water contains 1. 6. This means that growth is evenly distributed between foliage and roots. I will mix this with RO water until the mix is around 500 PPM and its ready for use. Following which, the eluate was further purified using mixed mode Oasis MCX SPE columns and this additional step helps to reduce matrix interference during MECC. It was found that trans-zeatin possessed an inhibitory effect against acetylcholinesterase (the enzyme involved in Alzheimer's disease) and might be used to treat Alzheimer's disease or related neural dysfunctions, such as dementia. Just like coconut fruit, coconut water offers us many health benefits. Note: Bananas are notorious for their high potassium counts, but just one cup of coconut water contains more potassium than a medium sized banana. Complete answer: Cytokinin is a coconut milk factor. MeSH terms High in potassium. One cytokinin known as trans-zeatin has been investigated as a possible new treatment for neuronal diseases including Alzheimer's disease. cytokinin. Calcium alone can't build strong bones, it needs phosphorus to help maximize its benefits. Each cytokinin or CW was used singly or in combina-tion. The optimum concentration of G3A2-ZR for cytokinin activity in the tobacco callus assay was estimated to be 5 x 10 (-6) M, so that G3A2-ZR is one order of magnitude more potent than 1,3-diphenylurea and one order less potent than zeatin riboside. Coconut milk is a rich source of cytokinin. . The liquid inside young coconut is often preferred to the liquid of a ripened coconut. With this function, where else in a plant would you expect to find cytokinin? The best CZE separation for the six cytokinin nucleotide standards was achieved using a 25 mM ammonium formate/formic acid buffer (pH 3.8) and 2% (v/v) methanol with an applied . cytokinin s in coconut (Cocos nucifera L.) . Build Stronger Bones and Teeth. @article{Ge2006AnalysisOC, title={Analysis of cytokinin nucleotides in coconut (Cocos nucifera L.) water using capillary zone electrophoresis-tandem mass spectrometry after solid-phase extraction. Jr. and O.K. [19] , whereas Liu et al. Cytokinins also promote seed germination and root cell division. so it is anti-ageing drink. Education. Natural Diuretic. Cytokines also help promote cell growth. The roles of cytokinin and gibberellic acid found in coconut water makes it the ideal organic fertilizer to facilitate the development of seedlings and clones of cannabis and this can be done with 15ml of coconut water per liter. In some other studies cytokinin supplementation was not always necessary for induction of SE (Jo and others 1996; Steinitz and others 2003). The invention relates to 6-substituted 9-halogenalkyl purine derivatives of the general formula I wherein R6 is selected from the group comprising NH-furfuryl, NH-(4-hydroxy warning . Kinetin was the first cytokinin discovered and so named because of the compounds ability to promote cytokinesis (cell division). Cytokinins had shown some significant anti-aging, anti-carcinogenic and anti-thrombotic effects that contributed to the various health benefits. If you really like the flavor, it can be a low-calorie, low-sugar alternative to soda. CW.

Answers (1) I infoexpert23 Ans. Int.J.Curr.Microbiol.App.Sci (2016) 5(4): 532-538 533 Another was some characterization of what the factor was at the chemical level.

This experiment is done with coconut water,water with tulsi leaves.

All the liquid media were adjusted to pH 5.7 prior to autoclaving at 1.07 kg/cm2, 120C for 15 min. Thus, the role of cytokinins in . Presence of trans-zeatin, a cytokinin, is very effective in Alzheimer's because it helps in preventing brain cell damage and also helps in curing impaired memory. Coconut water used in experiments was found to contain high level (>136 pmol ml(-1)) of zeatin riboside (ZR). Coconut water is known to enhance seed germination and sprouting of corms of Dracontium grayumianum (Torres et. trans-Zeatin riboside-5'-monophosphate (ZMP) was detected and quantified in coconut water by CZE-MS/MS after SPE and on-line sample stacking. It also has anti-bacterial and anti-fungal properties. Else the result would be as ,cytokinin has property of delaying senescence. Similar Questions P: Resistance of a conductor depends on its length Q. Cytokinins stimulate cell division, even in non-meristematic cells, in presence of auxin. Analysis of cytokinin nucleotides in coconut (Cocos nucifera L.) water using capillary zone electrophoresis-tandem mass spectrometry after solid-phase extraction. Coconut milk is the liquid endosperm in unripened fruit and is rich in plant hormone cytokinin. Shoots and roots were homogenized and cytokinins extracted in 80% ethanol. Cytokinin-like activity of coconut water was highest from 12-month old nuts and lowest from 8-month old nuts. Express 80050000000 in the standard or scientific form. 1 cup of coconut water can provide 10% of the daily requirement of vitamin C, 8% of Riboflavin, 5% of Thiamin and 4% of Vitamin B6. Author links open overlay panel Liya Ge a Jean Wan Hong Yong a Swee Ngin Tan a Xin Hao Yang . [19] , whereas Liu et al. So far, the application of CE for the identification of cytokinins in plant samples have been reported by Packov et al. I use a 3/8 inch drill bit that is over 3 inches long. Else the result would be as ,cytokinin has property of delaying senescence. Coconut water is one of the versatile and organically rich natural products in the . 1. All these vitamins are vital for the health of our body. trans-zeatin riboside is the most abundant type of cytokinin found in coconut water (Table 2). Kinetin, another cytokinin in coconut water, also possesses strong anti-cancer effects by inducing apoptosis (programmed cell death) in cancer cells. At least 20% of the cytokinin activity of coconut milk could be attributed to G 3 A 2 -ZR. It is prepared from selected coconuts and processed to remove most of the protein. According to Yong et al,(2009), the anti-inflammatory effect of coconut water might be due to its compositions of sugar, vitamins, minerals and cytokinins. Most people don't get enough potassium in their diet. Increased stamina: Coconut milk is rich in calories and electrolytes, which can improve stamina and reduce fatigue. Adenine, the N at the top is the N6 "side chain" that is an important part of making a cytokinin a cytokinin. Table 1 shows that compound 1 is cytokinin trans-zeatin riboside, a naturally occurring hormone involved with regulation of plant growth and development that was previously reported in coconut water (Ge et al., 2004, Kobayashi et al., 1995) and in coconut milk by Van Staden and Drewes (1975). Coconut milk also helps build muscle. Coconut water is the liquid endosperm, the actively dividing tissue which serves as nutrition source for developing coconut embryo. The cytokinins found in coconut water support cell division, and thus promote rapid growth. They may also play a significant role in the nutrition of plant and tissue cultures. The cultures were maintained on rotary shaker at 100 rpm at 272C under a 14-hr photoperiod with fluorescent lamps at 20 mmol/m2/ sec. Coconut water is rich in variety of vitamins such as Vitamin C (5.8 mg), Choline (2.6 mg), Niacin (0.2 mg), Riboflavin (0.1 mg), Thiamin (0.1 mg), etc. Coconut water contains natural cytokinins such as zeatin (Z) and related forms, kinetin (KIN), and N 6-( 2-isopentenyl)-adenine (iP)-like precursors (Ge and others 2006). The cytokinins in coconut water regulate cell division and influence the rate at which plants age. Coconut is also known specifically as buko juice. They called it cytokinin because it induced cytokinesis or cytoplasmic division.

Cytokinins are naturally occurring plant hormones that may help reduce the growth of cancer cells, although more research is needed at this time. In one study on tomato plants, scientists found that foliar growth nearly doubled and root growth nearly tripled when coconut water was used in watering . Solution. 1. Compounds 2, 3 and 6 are respectively a trimer . These hormones are normally synthesised in very small amounts by specialist cells . ( webmd.com) 4. The C18 solid-phase extraction (SPE) method was used to pre-concentrate the putative cytokinins present in the coconut water. Each cytokinin or CW was used singly or in combina-tion. CW. trans-zeatin is normally used to induce plantlet regeneration from callus in plant tissue culture.Based on experimental data, trans-zeatin plays a key role in the G 2-M transition of tobacco cells. If the leaves are degraded then it is because of more amount of nutrients present in coconut endosperm. Question 02: Coconut water contains (a) ABA (b) auxin (c) cytokinin (d) gibberellin. Coconut water has a low matter content (2% to 5% wet basis), mainly comprising sugars and minerals. So far, the application of CE for the identification of cytokinins in plant samples have been reported by Packov et al. Cytokinin purification and enzyme immunoassay were as described by Kudoyarova et al.. The isoprenoid cytokinin profiles in coconut showed a predominant pattern of 9 . High blood pressure over a long period of time can . You can reverse the age-clock by incorporating coconut water into your day-to-day beauty routine. Research has shown the two minerals are co-dependent for bone heath. Q. ABA 2. auxin 3. cytokinin 4. gibberellin Recommended MCQs - 100 Questions Plant Growth and Development Botany (2022) Practice questions, MCQs, Past Year Questions (PYQs), NCERT Questions, Question Bank, Class 11 and Class 12 Questions, NCERT Exemplar Questions and PDF Questions with answers, solutions, explanations, NCERT reference and difficulty level Coconut milk is the white flesh of the mature brown coconut that provides a number of health benefits. The combination of off-line SPE, on-line sample stacking and CZE-MS/MS approach was successfully applied to screen for endogenous cytokinin nucleotides present in coconut water sample. A diet rich in sodium can result in an increase in blood pressure. Therefore, the correct answer is C, i.e. This particular nut yielded 14oz of coconut water @ 2530 ppm / 5.8 ph. Acedo, A.L. Source. [20 . Endosperm tissues undergo one of three main modes of development, which are the nuclear, cellular and helobial modes [] and the development of coconut . A method based on solid-phase extraction (SPE) and capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) is described for the separation and determination of six cytokinin nucleotides in coconut water. Cytokinin profiling in plant tissues using ultra-performance liquid chromatography-electrospray tandem mass spectrometry. Coconut water contains natural auxins, cytokinins and other plant growth hormones. Since cytokinins can retard the effects of aging, they are known as anti-aging hormones. They are most abundant in the growing tissues which are active site of cell division, for example: root, embryos, fruits etc. . Quality criteria, such as the water per nut ratio, Total Soluble Solids (TSS), total sugar per nut, and [reducing sugars / total sugars] ratio, are good indicators for estimating the suitability of coconut cultivars for the . Coconut water contains (a) ABA (b) auxin (c) cytokinin (d) gibberellin Solution Coconut milk is the liquid endosperm in unripened fruit and is rich in plant hormone cytokinin. Jr. Bautista, O.K., (1985) Cytokinin-like and growth-promoting activity of coconut water as related to its effect on pole sitao pod deterioration Annals of Tropical Research Volume 8. Cytokinin. Cytokinins had shown some significant anti-aging, anti-carcinogenic and. The other hormones are absent in coconut milk. Depending on the amount of cytokinins present, the aging process in plants can be either accelerated or retarded. The first cytokinin, N6 -furfuryladenine (kinetin) was isolated from an autoclaved sample of herring sperm DNA in 1955 [ 22, 23 ]. Composition. This volume of coconut water can serve as the ideal organic solution for cloning and serve as an alternative to . March 2016 Available Online: 10 April 2016 Article Info . It was heat stable, non-volatile, water soluble, and definitely carbon-based amongst other characteristics. A hormone called cytokinin was discovered in coconut milk and found to stimulate cell division. [20 . By Ondej Novk . studied the effect of coconut water combined with cytokinin-group growth regulators on Dianthus caryophyllus L. by culturing meat on MS-added synthetic medium. Chemically, coconut water is composed of minerals, sugars, vitamins, amino acids . MS/MS with . The presence of these two cytokinins in coconut water showed its important role in the developing of coconut. trans-zeatin is the most abundant cytokinin in Arabidopsis, and the free base as well as the riboside and ribotide forms are shown.Kinetin is an artificial, aromatic cytokinin and benzyladenine is an example of . Cytokinin: A Plant Hormone. The cytokinins in coconut water regulate cell division and influence the rate at which plants age. Coconut water contains cytokinin hormones that also trigger the plants to divide their cells into growing shoots and roots, resulting in bushier growth. However, it should be noted that cytokinins cannot completely substitute coconut water's effects. Journal of Chromatography A, 2006. . (Coconut Water and Corn SST. Cytokinin biosynthesis happens through the biochemical modification of adenine. Read More Coconut water is a rich source of many different phytohormones, in particular, Cytokinins (Photo credit: Crisco 1492) Hormones can be defined as natural organic chemical messengers or signals that are produced by an organism to control the way its cells function. Depending on the amount of cytokinins present, the aging process in plants can be either accelerated or retarded. Coconut milk, especially the lower-fat variety, can be used in moderation (1-2 times per week). Solution: Cytokinins (Letham, 1963) are plant growth hormones which are basic in nature, either aminopurine or phenyl urea derivatives, that promote cytokinesis ( = cell division) either alone or in conjunction with auxin. All the liquid media were adjusted to pH 5.7 prior to autoclaving at 1.07 kg/cm2, 120C for 15 min. Reducing cytokinin production causes the . Bautista, (1984) Retardation of deteriotation in pole sitao with coconut water III Postharvest Research Notes Volume 1 Slow Down Aging, Cancer, and Alzheimer's. The other hormones are absent in coconut milk. Though it is a natural compound, It is not made in plants, and is therefore usually considered a "synthetic" cytokinin (meaning that the hormone is synthesized . Our data indicating the presence of cytokinin O-glucosides in coconut water will definitely be useful to researchers using coconut water as a growth supplement in tissue culture medium. Indonesia is the larger country with the largest coconut growing country in the world. Khatum et al. Question 02:Coconut water containsa ABAb auxinc cytokinind gibberellin. b. bacteria, improving the extraction of water and minerals from the soil c. fungi, storing food for the plant in the fungal bodies d. fungi . Coconut water contains (a) ABA (b) auxin (c) cytokinin (d) gibberellin Answer: (c) Coconut milk is the liquid endosperm in unripened fruit and is rich in plant hormone cytokinin. The cultures were maintained on rotary shaker at 100 rpm at 272C under a 14-hr photoperiod with fluorescent lamps at 20 mmol/m2/ sec. Coconut Water, Residual Coconut Water, Cytokinin, Auxin and Mineral Elements. The addition of cytokinin in plant's leaves increase transpiration which increases water loss. Phytohormones, particularly cytokinins, one of the most interesting components reported present in coconut water. }, author={Liya Ge and Jean W. H. Yong and Swee Ngin Tan and Xin Hao Yang and Eng Shi Ong}, journal={Journal of chromatography. al., 2011) [10] . Remaining protein levels in the water may vary from one lot to the next and may result in precipitate when . The optimum concentration of G 3 A 2 -ZR for cytokinin activity in the tobacco callus assay was estimated to be 510 6 M, so that G 3 A 2 -ZR is one order of magnitude more potent than 1,3-diphenylurea and one order less potent than zeatin riboside. They are mostly used to propagate protocorm-like bodies of orchids in plant industries [ 49 ]. This hormone helps in increasing the cell's growth, development, differentiation, affecting apical dominance, leaf senescence, and axillary bud growth. The Discovery process. Select one: a. bark . Coconut water is one of the best natural rejuvenants used in plant tissue culture as an organic supplement. Structures of cytokinins.Adenine is the parent compound of naturally occurring cytokinins, though it does not activate cytokinin responses; the N 6 position is indicated with an arrow. Publication types

Shekarriz et al . Concentrations of cytokinin (zeatin, its riboside, nucleotide, O-glucoside and N 9-glucoside) in the roots and shoots were determined 1 h and 6 h after the start of the experiment.